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阿拉丁核酸合成试剂
2020-06-23
寡核苷酸是短的、单链或双链的DNA或RNA分子。其合成方法发展历程：从Michelson和Todd早期的H-磷酸盐和磷酸三酯法（H-Phosphonate & Phosphotriester）；到1950年代Khorana的磷酸二酯法（Phosphodiester）；以及1960-1970年代的磷酸三酯法（Phosphotriester）和亚磷酸三酯法（Phosphite triester）的发展。而直至1980年代，Marvin Caruthers的亚磷酰胺法（Phosphoramidite），加上自动化固相技术，成为了目前主流的寡核苷酸合成技术方法。在聚合酶链反应(PCR)、核酸测序、基因检测、核酸药物研发等领域有广泛应用。
亚磷酰胺寡聚体的合成在3'-5'方向进行(与生物合成中DNA复制的5'-3'方向相反)。每个合成周期添加一个核苷酸。亚磷酰胺DNA合成循环如图所示。
亚磷酰胺DNA合成循环
保护核苷
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| I119475 | N2-异丁酰基-2′-脱氧鸟苷 | N2-Isobutyryl-2′-deoxyguanosine | 98% | 68892-42-2 | 100mg,250mg,1g,5g |
| I119519 | 5'-O-(4,4'-二甲氧基三苯基)-N2-异丁酰基-2'-脱氧鸟苷 | N2-Isobutyryl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyguanosine | 99.00% | 68892-41-1 | 1g,5g,10g,25g,100g |
| N159691 | N6-二苯甲酰基腺苷-2',3'-二苯甲酸酯 | N6-Dibenzoyladenosine 2',3'-Dibenzoate | >98.0%(HPLC)(T) | 58463-04-0 | 100mg,1g |
| B119471 | N4-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脱氧胞苷 | N4-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxycytidine | 99% | 67219-55-0 | 1g,5g |
| B103100 | N4-苯甲酰胞苷 | N4-Benzoylcytidine | 98% | 13089-48-0 | 1g,5g,25g |
| B119532 | N4-苯甲酰基-2'-脱氧胞苷 | N4-Benzoyl-2′-deoxycytidine | 98.00% | 4836-13-9 | 250mg,1g,5g |
| B122954 | N6-苯甲酰基-2'-脱氧腺苷水合物 | N6-Benzoyl-2′-deoxyadenosine hydrate | 98% | 206752-42-3,4546-72-9 | 250mg,1g,5g |
| B119470 | N6-苯甲酰基-5′-O-(4,4′-二甲氧基三苯基)-2′-脱氧腺苷 | N6-Benzoyl-5′-O-(4,4′-dimethoxytrityl)-2′-deoxyadenosine | 99% | 64325-78-6 | 100mg,1g,5g |
| O117684 | 5′-O-(4,4′-二甲氧基三苯甲基)胸苷 | 5′-O-(4,4′-Dimethoxytrityl)thymidine | 98.00% | 40615-39-2 | 1g,5g,25g |
| B119477 | N6-苯甲酰基腺苷 | N-Benzoyladenosine | 97% | 4546-55-8 | 1g,5g,25g |
核糖，2'-脱氧核糖
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| R105972 | L-核糖 | L-(+)-Ribose | 98% | 24259-59-4 | 1g,5g,10g,25g,100g |
| T107915 | 四乙酰核糖 | 1,2,3,5-Tetra-O-acetyl-β-D-ribofuranose | 98% | 13035-61-5 | 5g,25g,100g,500g |
| R104821 | D-(-)-核糖 | D-(-)-Ribose | ≥99%（HPLC) | 50-69-1 | 5g,25g,100g,250g,500g |
| R104820 | D-(-)-核糖 | D-(-)-Ribose | 98% | 50-69-1 | 100g,500g,2.5Kg |
| R103230 | 1-乙酰基-2,3,5-三苯甲酰氧基-1-beta-D-呋喃核糖 | β-D-Ribofuranose 1-acetate 2,3,5-tribenzoate | 98% | 6974-32-9 | 1g,5g,25g,100g |
| M158368 | 甲基-β-D-呋喃核糖苷 | Methyl β-D-Ribofuranoside | >97.0%(HPLC) | 7473-45-2 | 1g,5g,25g,100g |
| D121077 | 3,5-二-O-(对甲苯酰)-2-脱氧-Α-D-呋喃核糖氯 | 3,5-Di-O-(p-toluyl)-2-deoxy-D-ribofuranosyl chloride | 90% | 4330-21-6 | 250mg |
| D104784 | 2-脱氧-D-核糖 | 2-Deoxy-D-Ribose | 98% | 533-67-5 | 1g,5g,25g,100g |
| D123989 | 2-脱氧-2,2-二氟-D-赤-戊糖酸γ-内酯3,5-二苯甲酸酯 | 2-Deoxy-2,2-difluoro-D-erythro-pentonic Acid γ-Lactone 3,5-Dibenzoate | 98% | 122111-01-7 | 1g,5g,25g,100g |
| T139496 | 2-脱氧-2-氟-1,3,5-三-O-苯甲酰基-α-D-阿拉伯呋喃糖 | 2-Deoxy-2-fluoro-1,3,5-tri-O-benzoyl-α-D- arabinofuranose | - | 97614-43-2 | 1g,5g,25g |
硫转移试剂
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| X162986 | 氢化黄原素 | Xanthane Hydride | >95.0%(HPLC) | 6846-35-1 | 5g,25g,100g,500g |
| H157193 | 3H-1,2-苯并二硫醇-3-酮1,1-二氧化物 | 3H-1,2-Benzodithiol-3-one 1,1-Dioxide | >98.0%(HPLC) | 66304-01-6 | 250mg,1g,5g |
| B152485 | 双(苯乙酰)二硫化物 | Bis(phenylacetyl) Disulfide | >98.0%(HPLC) | 15088-78-5 | 5g,25g |
磷酸化试剂，磷硫化试剂
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| M157887 | 二氯磷酸甲酯 | Methyl Phosphorodichloridate | >95.0%(T) | 677-24-7 | 5g,25g,100g |
| C119480 | 2-氰乙基N,N,N′,N′-四异丙基亚磷酰二胺 | 2-Cyanoethyl N,N,N′,N′-tetraisopropylphosphordiamidite | 97% | 102691-36-1 | 1g,5g,25g,100g |
| T162687 | 焦磷酸四苄酯 | Tetrabenzyl Pyrophosphate | >98.0%(HPLC) | 990-91-0 | 250mg,1g,5g,25g |
| P104277 | 二氯化磷酸苯酯 | Phenyl dichlorophosphate | 98% | 770-12-7 | 25g,100g,500g |
| B152354 | 二(四丁铵)焦磷酸二氢盐 | Bis(tetrabutylammonium) Dihydrogen Pyrophosphate | >96.0%(T) | 857447-79-1 | 50mg,200mg |
| H157193 | 3H-1,2-苯并二硫醇-3-酮1,1-二氧化物 | 3H-1,2-Benzodithiol-3-one 1,1-Dioxide | >98.0%(HPLC) | 66304-01-6 | 250mg,1g,5g |
| C135875 | 2-氯-1,3,2-二氧磷杂环戊烷 2-氧化物 | 2-Chloro-1,3,2-dioxaphospholane-2-oxide | ≥95.0% | 6609-64-9 | 5g,25g,100g |
| D156020 | 氯硫代磷酸二乙酯 | Diethyl Chlorothiophosphate | >97.0%(GC) | 2524-04-1 | 5g,25g,100g |
| C154011 | 2-氯苯基二氯膦酸酯[磷酸化剂] | 2-Chlorophenyl Phosphorodichloridate [Phosphorylating Agent] | >97.0%(T) | 15074-54-1 | 1g,5g,25g,100g |
| C154013 | 2-氯-4H-1,3,2-苯并二氧磷-4-酮 | 2-Chloro-4H-1,3,2-benzodioxaphosphorin-4-one | >95.0% | 5381-99-7 | 1g,5g,25g |
| D109475 | 氯代磷酸二苯酯 | Diphenyl phosphoryl chloride | 97% | 2524-64-3 | 25g,100g,500g |
| M157888 | 二氯亚磷酸甲酯[亚磷酸化剂] | Methyl Dichlorophosphite [Phosphitylating Agent] | >95.0%(T) | 3279-26-3 | 5g,10g,25g,100g |
| B152485 | 双(苯乙酰)二硫化物 | Bis(phenylacetyl) Disulfide | >98.0%(HPLC) | 15088-78-5 | 5g,25g |
| C154012 | 4-氯苯基二氯磷酸酯 | 4-Chlorophenyl Phosphorodichloridate | >97.0%(T) | 772-79-2 | 5g,25g,100g |
| D110276 | 焦磷酰氯 | Diphosphoryl chloride | 97% | 13498-14-1 | 5g,25g,100g,500g |
| C153709 | 5-氯水杨基-N,N-二异丙基亚磷酰胺 | 5-Chlorosaligenyl-N,N-diisopropylphosphoramidite | >95.0%(HPLC) | 1620086-77-2 | 200mg,1g |
| C154014 | 2-氯-1,3,2-二氧磷杂环戊烷 | 2-Chloro-1,3,2-dioxaphospholane | >97.0%(GC) | 822-39-9 | 5g,25g,100g |
| D138186 | N,N-二异丙基亚磷酰胺二叔丁酯 | Di-tert-butyl N,N-diisopropylphosphoramidite | ≥95.0% | 137348-86-8 | 1g,5g |
| D122720 | 二苄基 N,N-二异丙基亚磷酰胺 | Dibenzyl N,N-Diisopropylphosphoramidite | 98% | 108549-23-1 | 1g,5g,25g,100g |
缩合剂
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| N132858 | 3-硝基-1,2,4-三唑 | 3-Nitro-1,2,4-triazole | ≥98.0%(HPLC) | 24807-55-4 | 1g,5g,25g,100g |
| M121621 | 均三甲苯基磺酰氯 | 2-Mesitylenesulfonyl chloride | 99% | 773-64-8 | 5g,25g,100g,500g |
| C113337 | N,N'-羰基二(1,2,4-三氮唑) | 1,1'-Carbonyl-di-(1,2,4-triazole) | 96% | 41864-22-6 | 5g,25g,100g |
| M135672 | 2,4-二磺酰氯基均三甲苯 | 2,4-Mesitylenedisulfonyl Dichloride | ≥98.0% | 68985-08-0 | 5g,25g |
| T109597 | 特戊酰氯 | Trimethylacetyl chloride | 98% | 3282-30-2 | 25ml,100ml,500ml,2.5L,10L |
| B115197 | 5-苄硫基四氮唑 | 5-(Benzylthio)-1H-tetrazole | 98.50% | 21871-47-6 | 5g,25g,100g |
| D106074 | N,N'-二环己基碳二亚胺(DCC) | N,N′-Dicyclohexylcarbodiimide | 99.00% | 538-75-0 | 25g,100g,500g,1kg,2.5kg,10kg |
| C153419 | 1-(氰甲基)哌啶四氟硼酸盐 | 1-(Cyanomethyl)piperidinium Tetrafluoroborate | >98.0%(N) | 434937-12-9 | 1g,5g |
| T113353 | 1-(2,4,6-三异丙基苯基磺酰)咪唑 | 1-(2,4,6-Triisopropylbenzenesulfonyl)imidazole | 98% | 50257-40-4 | 1g,5g,10g,25g |
| D109319 | 4,5-二氰基咪唑 | 4,5-Dicyanoimidazole | 98% | 1122-28-7 | 5g,25g,100g |
| D109320 | 4,5-二氰基咪唑 | 4,5-Dicyanoimidazole | 99% | 1122-28-7 | 5g,25g,100g |
| T161612 | 2,4,5,6-四甲基苯二磺酰二氯 | 2,4,5,6-Tetramethylbenzenedisulfonyl Dichloride | >98.0%(T) | 97997-76-7 | 1g,5g |
| T138965 | 1-(2,4,6-三异丙基苯磺酰)-1,2,4-三唑 | 1-(2,4,6-Triisopropylphenylsulfonyl)-1,2,4-triazole | >98.0%(HPLC) | 54230-60-3 | 1g,5g,25g |
| T106183 | 1-对甲基苯磺酰咪唑 | 1-(p-Toluenesulfonyl)imidazole | 99% | 2232-08-8 | 5g,25g,100g,500g |
| T109341 | 2,4,6-三异丙基苯磺酰氯 | 2,4,6-Triisopropylbenzenesulfonyl chloride | 97% | 6553-96-4 | 5g,25g,100g,500g |
| D105651 | N,N'-二环己基-4-吗啉脒 | N,N′-Dicyclohexyl-4-morpholinecarboxamidine | 98% | 4975-73-9 | 5g,25g,100g,500g |
| T100645 | 1,2,4-三氮唑 | 1,2,4-Triazole | 99% | 288-88-0 | 25g,100g,250g,500g |
| E111310 | 5-乙硫基四氮唑 | 5-(Ethylthio)-1H-tetrazole | 98% | 89797-68-2 | 1g,5g,25g,100g |
羟基保护剂，氨基保护剂
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| I107549 | 异丁酰氯 | Isobutyryl Chloride | 98% | 79-30-1 | 25ml,100ml,500ml,2.5L |
| H106017 | 六甲基二硅胺烷 | Hexamethyl disilylamine | AR,98% | 999-97-3 | 100ml,500ml,2.5L,10L |
| D154192 | 二叔丁基二氯硅烷 | Di-tert-butyldichlorosilane | >95.0%(GC) | 18395-90-9 | 1g,5g |
| N106603 | 2-硝基溴化苄 | 2-Nitrobenzyl bromide | 97% | 3958-60-9 | 5g,25g,100g |
| P160117 | 苯氧基乙酰氯 | Phenoxyacetyl Chloride | >98.0%(GC) | 701-99-5 | 25g,100g,500g |
| B152896 | 1,3-苯并二硫代吡咯四氟化硼盐[羟基保护剂] | 1,3-Benzodithiolylium Tetrafluoroborate [Hydroxyl-Protecting Agent] | >96.0%(T) | 57842-27-0 | 1g,5g |
| T109041 | N,O-双(三甲基硅烷基)乙酰胺 | N,O-Bis(trimethylsilyl)acetamide | 95% | 10416-59-8 | 25ml,100ml,500ml,5L |
| T131644 | N,O-双(三甲基硅烷基)乙酰胺 | TMS-BA | 用于GC衍生化 | 10416-59-8 | 5ml,25ml |
| A108664 | 乙酰氯 | Acetyl chloride | 99%,无色 | 75-36-5 | 500ml,2.5L |
| A108662 | 乙酰氯 | Acetyl chloride | AR,98% | 75-36-5 | 100ml,500ml,12*500ml,2.5L,10L |
| C134968 | 2-氯代三苯甲基氯 | 2-Chlorotrityl Chloride | ≥97.0% | 42074-68-0 | 25g,100g,500g,2.5kg |
| T109597 | 特戊酰氯 | Trimethylacetyl chloride | 98% | 3282-30-2 | 25ml,100ml,500ml,2.5L,10L |
| D106289 | N,N-二甲基甲酰胺二甲缩醛 | DMF-DMA | 98%,用于酯化 | 4637-24-5 | 25g,100g,500g |
| D106288 | N,N-二甲基甲酰胺二甲缩醛 | N,N-Dimethylformamide Dimethyl Acetal | 97% | 4637-24-5 | 25ml,100ml,500ml |
| T106184 | 三苯基氯甲烷 | Trityl Chloride | 97% | 76-83-5 | 25g,100g,250g,500g,2.5kg |
| C153889 | 4-氯苯氧基乙酰氯 | 4-Chlorophenoxyacetyl Chloride | >98.0%(T) | 4122-68-3 | 5g,10g,25g,100g,500g |
| T109341 | 2,4,6-三异丙基苯磺酰氯 | 2,4,6-Triisopropylbenzenesulfonyl chloride | 97% | 6553-96-4 | 5g,25g,100g,500g |
| D109292 | 4,4'-二甲氧基三苯基氯甲烷 | 4,4′-Dimethoxytrityl chloride | >97.0% | 40615-36-9 | 5g,25g,100g,500g |
| D119481 | 二叔丁基硅基双(三氟甲烷磺酸) | Di-tert-butylsilyl bis(trifluoromethanesulfonate) | 97% | 85272-31-7 | 1g,5g,25g |
| B111191 | 叔丁基二苯基氯硅烷 | tert-Butyldiphenylchlorosilane | 98% | 58479-61-1 | 10g,25g,100g,250g |
| B103644 | 叔丁基二甲基氯硅烷 | tert-Butyldimethylsilyl chloride | 97% | 18162-48-6 | 25g,100g,250g,500g |
| T162634 | 4,4',4''-三(苯酰氧基)三苯甲基溴[羟基保护剂] | 4,4',4''-Tris(benzoyloxy)trityl Bromide [Hydroxyl Protecting Agent] | >97.0%(T) | 86610-66-4 | 1g,5g,25g |
| D113529 | 1,3-二氯四甲基二硅氧烷 | 1,3-Dichloro-1,1,3,3-tetramethyldisiloxane | 95% | 2401-73-2 | 5g,25g,100g |
| D139183 | 1,3二氯-1,1,3,3-四异丙基二硅氧烷 | 1,3-Dichloro-1,1,3,3-tetraisopropyldisiloxane | ≥97% | 69304-37-6 | 1g,5g,25g,100g |
| M100611 | 4-甲氧基苯甲酰氯 | 4-Methoxybenzoyl chloride | 97% | 100-07-2 | 25g,100g,500g |
| B104566 | 苯甲酰氯 | Benzoyl chloride | ACS, ≥99.0% (T) | 98-88-4 | 500g |
| C104814 | 三甲基氯硅烷(TMCS) | Chlorotrimethylsilane | ≥99.0%(GC) | 75-77-4 | 100ml,250ml,500ml,2.5L,10L |
| C104813 | 三甲基氯硅烷(TMCS) | Chlorotrimethylsilane | >98.0%(GC) | 75-77-4 | 100ml,250ml,500ml,2.5L,10L |
| T106616 | 三氟甲磺酸三甲基硅酯 | Trimethylsilyl trifluoromethanesulfonate | 98.00% | 27607-77-8 | 5g,10g,25g,50g,100g,250g,500g |
| M113503 | 4-甲氧基氯化三苯甲烷 | 4-Monomethoxytrityl chloride | 97% | 14470-28-1 | 5g,10g,25g,100g,250g |
| B104818 | 苯甲酸酐 | Benzoic anhydride | 98% | 93-97-0 | 25g,100g,500g |
脱保护剂
| 产品号 | 中文名称 | 英文名称 | 级别 | Cas | 规格 |
|---|---|---|---|---|---|
| T103291 | 三氟乙酸（TFA） | Trifluoroacetic acid | AR,99.0% | 76-05-1 | 100g,500g,2.5kg |
| H107501 | 六氟异丙醇 | 1,1,1,3,3,3-Hexafluoro-2-propanol | 99.50% | 920-66-1 | 25g,100g,250g,500g,2.5kg |
